June
11 |
|
8:30-8:45 a.m. |
Lecture: Welcome and Introduction to the
Downstream Processing and Protein Purification
Workshop |
| 8:45-9:45
a.m. |
Lecture: Overview
of Downstream Processing |
| 9:45-10:30
a.m. |
Lecture: Current
Options for Cell Separation |
| 10:30-10:45
a.m. |
Break |
| 10:45
a.m. -12:00 p.m. |
Lecture: Cross
Flow Filtration |
| 12:00
-1:00 p.m. |
Lunch |
| 1:00-1:45
p.m. |
Lecture/Lab: Cell
Separation by Centrifuge abd TFF |
| 1:45-2:30
p.m. |
Lecture/Lab: Cell
Disintegration, Methods and Demonstration |
| 2:30-4:00
p.m. |
Lab: Protein
Concentration and Buffer Exchange Using TFF |
| |
|
June
12 |
|
| 8:45-9:15
a.m. |
Lecture: Gel
Filtration/Size Exclusion Chromatography |
| 9:15-10:00
a.m. |
Lecture: Ion
exchange Chromatography |
| 10:00-10:15
a.m. |
Break |
| 10:15-11:00
a.m. |
Lecture: Hydrophobic
Interaction Chromatography |
| 11:00
a.m.-12:00 p.m. |
Lecture: Affinity
Chromatography |
| 12:00-1:00
p.m. |
Lunch |
| 1:00-2:15
p.m. |
Lecture: Purification
Strategies
|
| 2:15-2:30
p.m. |
Break
|
| 2:30-4:30
p.m. |
Lab: Sample
Prep-Desalting Parameters
|
| |
|
June
13 |
|
| 8:30-10:00
a.m. |
Lecture: Process
Optimization
|
| 10:00-10:15
a.m. |
Break |
| 10:15-11:15
a.m. |
Lecture: Process
Hygiene |
| 11:15
a.m.-12:00 p.m. |
Lecture: Scale-Up |
| 12:00-1:00
p.m. |
Lunch |
| 1:00-1:30
p.m. |
Lecture: Scale-Up,
continued |
| 1:30-1:45
p.m. |
Break |
| 1:45-4:00
p.m. |
Lecture/Lab: Introduction
to Membrane Filtration |
| 4:00-4:30
p.m. |
Wrap-up: Questions
and Answers |
