Lecture topics include:
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cell physiology and medium components
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fermentors and accessories, probes and instrumentation
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recombinant E. coli protein expression and fermentation
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oxygen consumption and transfer data analysis
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scale-up of fermentations: principles, practice and problems
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case studies illustrating how principles are applied in practice.
Laboratory
sessions include:
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assembly and disassembly of bench top fermentors (2-5 liter) from several different vendors
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sterilization procedures
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preparation of inocula and inoculation
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process sampling
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measurement of optical density and glucose levels
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process control
- harvesting techniques
Workshop
laboratory sessions
The workshop laboratory sessions take place in a newly renovated learning facility located within the University of Maryland Bioprocess Scale-up Facility (BSF) on the College Park campus. The BSF houses bioprocessing equipment for process development and optimization ranging from 1 L to 280 L. All workshop participants will examine and test large-scale fermentation equipment while they perform their own experiments on bench-top units provided by several vendors. The format is fast-paced, but plenty of time has been allocated for discussion with workshop faculty.
Case Studies: In response to requests from past attendees, a series of case studies are being added to the curriculum so that participants may gain a greater understanding of how the principles of fermentation are applied in practice. Please see the complete workshop schedule for more details.
Cost
Two-day workshop $995
Fermentation and Downstream Workshops (5 days) $1,971
Lodging is not included in registration fee.
Meals
Continental breakfasts, catered lunches and coffee
breaks will be provided, and are included in the registration
fee.
