ENES 489Q: Biotechnology/Bioproduction
Course Syllabus
Course Name: Biotechnology / Bioproduction
Course Number: ENES 489Q
Credits: 3
Prerequisites: BSCI 223 or CHEM 231/232; Exceptions with permission of the instructors.
Instructor:
Ben Woodard
Manager, Bioprocess Scale-up Facility
1211 Chemical Engineering Bldg. #090
Office hours: M-F 9:30 AM – 12:00 PM
Phone: 301 405-3909
Course Description: This course teaches the basics of recombinant DNA technology and biopharmaceutical manufacturing:
- Genetic engineering & gene splicing
- Current & future trends in biotechnology
- Creating and maintaining the cell bank
- Culture media
- Inoculum development
- Bioreactors
- Process control strategy
- Cell harvesting
- Product recovery & purification
- Process chromatography
- Product concentration
- Formulation
- Fill & finish
Course Schedule: This 3-credit Winter Term course will meet five afternoons per week, for three weeks from January 3 through January 23, 2006, except Martin Luther King Holiday January 16, 2006.
Course Structure: Instruction will combine classroom lectures and practical laboratory exercises. Students will work through a “production campaign” from expression system to vialed protein product. The “hands on” experience will be especially valuable to students interested in the growing field of biopharmaceutical production.
Course Policies: Students missing more than one lecture and one lab will receive an incomplete grade. There will be a 15 minute grace period for late arrivals due to weather conditions only. Classes, quizzes, lab practicals and exams missed due to Campus closure will be made up the following morning beginning at 9:00 AM. Lectures are structured around student participation. All students are expected to take part in discussions and play an active role during laboratory exercises.
Texts: "Bioprocess Engineering, Basic Concepts," 2nd Edition, Michael L. Shuler and Fikret Kargi, Prentice Hall, 2001. ISBN: 0-13-081908-5. Course notes.
Additional Materials: Students must supply lab coat, safety eye glasses, laboratory notebook.
Lecture Schedule (UNDER DEVELOPMENT)
January 2006
3 Origins of Biotechnology, Biotechnology Industry
4 Expression, Cell Banks
5 Media Development
6 Inoculum Development
9 Bioreactors, Controls
10 Scale-up
11 Quiz, Operating Modes
12 Separations, Lysis
13 Documentation
16 Holiday
17 Chromatography
18 Concentration
19 Final Processing Steps
20 Laboratory Final, no lecture
23 Exam
Lab schedule
3 No lab
4 Aseptic Technique, Understanding SOP's, Cell Counting, Staining
5 Expression, Bacterial Transformation
6 Media Optimization, Growth Curves
9 Cell Banks and Bioreactor Design
10 Cell Culture, Eukaryotic Scale-Up
11 Fermentation, Prokaryotic Scale-Up
12 Cell Separations & Cell Disruption
13 Ion exchange Chromatography
16 Holiday
17 HIC Chromatography
18 Pilot Scale Chromatography
19 Ultrafiltration & Diafiltration
20 Laboratory Final
23 Final Exam, no lab
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